| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.208896 |
| Chromosome: | chromosome 6 |
| Location: | 2551868 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g269300 | CGL120 | (1 of 1) PF07103 - Protein of unknown function (DUF1365) (DUF1365); Conserved in the Green Lineage 120 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGTGTGGGCTGGGTGAGTGGGTGGCTAG |
| Internal bar code: | ACTTTGATAATATACAAGGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 473 |
| LEAP-Seq percent confirming: | 99.8389 |
| LEAP-Seq n confirming: | 4338 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGAATTCCGGTGGGTGTA |
| Suggested primer 2: | GTACACGCTGATGGGGTTCT |