| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208904 |
| Chromosome: | chromosome_2 |
| Location: | 1404471 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g083354 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TATGAAATCACCTGAACATGAACACACCAA |
| Internal bar code: | TCGGCGGAACATCCCAGCAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 368 |
| LEAP-Seq percent confirming: | 95.9477 |
| LEAP-Seq n confirming: | 734 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAAAGACGATGGAACCT |
| Suggested primer 2: | AAGAAGCAGCGAAAGCGTAG |