| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.208914 |
| Chromosome: | chromosome 16 |
| Location: | 1410927 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652250 | CMA3 | Chlamydomonas-specific membrane protein family A | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCCCTGTTCTCATTGTACTAGTCAGGCA |
| Internal bar code: | GTGGTCCGTACCGCGCCTTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 98 |
| LEAP-Seq percent confirming: | 99.3333 |
| LEAP-Seq n confirming: | 149 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATAGTTGAGGTGCATGGGC |
| Suggested primer 2: | AACCCAACTGACCGTACCAG |