| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.208916 |
| Chromosome: | chromosome 17 |
| Location: | 273991 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g698150 | CYCR4 | Cyclin-related protein; (1 of 10) PTHR10177 - CYCLINE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGTGGGATACGTGCAAACATGTTGGGCT |
| Internal bar code: | TCATGCTATACCAGTAGTCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 232 |
| LEAP-Seq percent confirming: | 99.7122 |
| LEAP-Seq n confirming: | 1386 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGGAGTTGGCGATAGGTA |
| Suggested primer 2: | GAGACAGGGTAAGTCGCTGC |