| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.208921 |
| Chromosome: | chromosome 12 |
| Location: | 2781138 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g502900 | (1 of 3) 3.4.24.13 - IgA-specific metalloendopeptidase / Immunoglobulin A1 proteinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAGCAGCAAACTTTTGGAATGCAAAGAT |
| Internal bar code: | AGGAGGAGCGAAGGGGGGGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 650 |
| LEAP-Seq percent confirming: | 99.1197 |
| LEAP-Seq n confirming: | 563 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAATGTGGTGAGAACGTG |
| Suggested primer 2: | CAGCAGAGTGACAGAGCAGG |