| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.208970 |
| Chromosome: | chromosome 7 |
| Location: | 376589 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g314800 | Coiled-Coil Protein; (1 of 140) IPR002048 - EF-hand domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAAGCTGCTGGGTGGGTGCAGTGCTGGT |
| Internal bar code: | TGGGTGTCGCCGACTGAACGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 556 |
| LEAP-Seq percent confirming: | 84.8039 |
| LEAP-Seq n confirming: | 1211 |
| LEAP-Seq n nonconfirming: | 217 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAACCGCTCAATCTTAAA |
| Suggested primer 2: | GACGTTGGTGGTCGCTATTT |