| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.208975 |
| Chromosome: | chromosome 3 |
| Location: | 4193564 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g174000 | EFG11,LPA2 | Mitochondrial translation elongation factor EFG/EF2, LepA-related; (1 of 1) PTHR23115:SF97 - TRANSLATION FACTOR GUF1, MITOCHONDRIAL | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCGTAGGAGCTGAGCGAGATGTGAAATT |
| Internal bar code: | AATAAACCTGCGGGCGCGCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 804 |
| LEAP-Seq percent confirming: | 87.3682 |
| LEAP-Seq n confirming: | 5139 |
| LEAP-Seq n nonconfirming: | 743 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCATAACAACCTTGGCCC |
| Suggested primer 2: | CTCGTTCGACTACGAGGAGG |