| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208984 |
| Chromosome: | chromosome 16 |
| Location: | 7739589 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g691776 | (1 of 2) K03145 - transcription elongation factor S-II (TFIIS) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTCAGGTTCAGCGTTAGGAGAGTTTGGT |
| Internal bar code: | GCGTTGGACCCAACGCCGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 264 |
| LEAP-Seq percent confirming: | 86.8881 |
| LEAP-Seq n confirming: | 3976 |
| LEAP-Seq n nonconfirming: | 600 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACCTTGAGGCTGGCTAC |
| Suggested primer 2: | TCTTGCATCAACAACGCTTC |