Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.208986 |
Chromosome: | chromosome 10 |
Location: | 1445593 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g428550 | DTS1 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCACCACCACCCGCCTTCAGACCCGGC |
Internal bar code: | TTTCGCGGCGGCGACAGGAACT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 848 |
LEAP-Seq percent confirming: | 99.6764 |
LEAP-Seq n confirming: | 1232 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATTACGAAGGTCTGGGGCTT |
Suggested primer 2: | CATCCACACATGCAAGTTCC |