| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208997 |
| Chromosome: | chromosome 17 |
| Location: | 5878553 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g739900 | (1 of 1) IPR012337//IPR027417 - Ribonuclease H-like domain // P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGATGAGGCTTGCTAACGCTGTGGCCGCC |
| Internal bar code: | CGAGAGCAGCACGGTCAAATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 664 |
| LEAP-Seq percent confirming: | 97.7198 |
| LEAP-Seq n confirming: | 11314 |
| LEAP-Seq n nonconfirming: | 264 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAAGTGTGTTGACGCAAG |
| Suggested primer 2: | CTACACACCATGCGGTCATC |