| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.209019 |
| Chromosome: | chromosome 2 |
| Location: | 7136547 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146629 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGGCCGCCGGGGACCTGGGTATGGGCAG |
| Internal bar code: | GTCTACTACGATAATTCGAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 573 |
| LEAP-Seq percent confirming: | 77.6923 |
| LEAP-Seq n confirming: | 101 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCACTCCCAACGTATGTC |
| Suggested primer 2: | CTCGAGTACTTGGAGGCAGG |