| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.209056 |
| Chromosome: | chromosome 5 |
| Location: | 1790770 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g242301 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACATTCTTGGAGACACGGCCACGGACTCG |
| Internal bar code: | GGTACCGGGTTTACCCGGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 962 |
| LEAP-Seq percent confirming: | 99.5531 |
| LEAP-Seq n confirming: | 16486 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAAGTGCTTGGCTCAGAC |
| Suggested primer 2: | ATGAGGCGCTTCAAGACACT |