| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.209069 |
| Chromosome: | chromosome 11 |
| Location: | 1782484 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467400 | (1 of 3) IPR000104//IPR001859 - Antifreeze protein, type I // Trypanosoma cruzi ribosomal protein P2-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGCGGCCCCCACGCACGCGCCGCATTA |
| Internal bar code: | CCCGTGGCAGTTACCCGTGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 566 |
| LEAP-Seq percent confirming: | 99.2344 |
| LEAP-Seq n confirming: | 5444 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGTACGTATCACTGCCG |
| Suggested primer 2: | AGAGCGACGACGACTTTCAC |