| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.209080 |
| Chromosome: | chromosome 3 |
| Location: | 7421029 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206000 | (1 of 1) PTHR16301//PTHR16301:SF2 - IMPACT-RELATED // PROTEIN IMPACT HOMOLOG | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCTGAATGCTTCTGCTACACTTGCAGGC |
| Internal bar code: | GGTGCCGCCTTAGTATAGGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 722 |
| LEAP-Seq percent confirming: | 99.2908 |
| LEAP-Seq n confirming: | 700 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGCAGTTCTTGTTCAGGC |
| Suggested primer 2: | TGACTACACACGCGCATACA |