| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.209091 |
| Chromosome: | chromosome 6 |
| Location: | 5562521 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g285350 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGTCGTAAATCAGGTCTCTGAGTTGGCA |
| Internal bar code: | TCAAAATCCGAGTATTGTCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 659 |
| LEAP-Seq percent confirming: | 99.62 |
| LEAP-Seq n confirming: | 3408 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACCTTGATAGACGGGC |
| Suggested primer 2: | GAACGACGCTATGAAGAGGC |