Insertion junction: LMJ.RY0402.209096_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.209096
Chromosome:chromosome 17
Location:3344125
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g723450 FAP13 WD-repeat Flagellar Associated Protein 13; (1 of 1) IPR001680//IPR007111//IPR017986//IPR020472//IPR027417 - WD40 repeat // NACHT nucleoside triphosphatase // WD40-repeat-containing domain // G-protein beta WD-40 repeat // P-loop containing nucleoside triphosphate hydrolase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTCACCACGGGCGCCGCGGGCGCCGGAG
Internal bar code:CGGTCGATCGTCGCCCGGTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:956
LEAP-Seq percent confirming:99.9032
LEAP-Seq n confirming:4129
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:AACCACCTCGTTTGTCCTTG
Suggested primer 2:GGCGGCTGGATAATCAGATA