Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.209147 |
Chromosome: | chromosome 7 |
Location: | 178939 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g313400 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTGACAACGCCAGCCGCAAGCGAGGCG |
Internal bar code: | AATGGCCCAGGAAACATAACGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 278 |
LEAP-Seq percent confirming: | 93.3665 |
LEAP-Seq n confirming: | 1689 |
LEAP-Seq n nonconfirming: | 120 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACACACACACACACACACGC |
Suggested primer 2: | CGCAAGAACATTTCCCACTT |