| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.209167 |
| Chromosome: | chromosome 12 |
| Location: | 7464029 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556250 | SEP1 | Septin-like protein; (1 of 1) K16944 - septin 7 (SEPT7, CDC3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGTTGTAGACTGGCTGGGGGAGTAAAG |
| Internal bar code: | TGTTGATAGCTCGGAGGTCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 461 |
| LEAP-Seq percent confirming: | 99.7803 |
| LEAP-Seq n confirming: | 3179 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCATTACCCTGACCCTGT |
| Suggested primer 2: | GGAAGCCACCTGAGACAGAG |