| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.209170 |
| Chromosome: | chromosome 12 |
| Location: | 2986133 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g501150 | MLT2 | (1 of 31) IPR003590 - Leucine-rich repeat, ribonuclease inhibitor subtype; Multi eye protein 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCACGTACCGCGCCCCTGCGCCCGCCGTG |
| Internal bar code: | ATGTAGTCAAAGCAACGATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 771 |
| LEAP-Seq percent confirming: | 99.7501 |
| LEAP-Seq n confirming: | 7984 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGACCGTCAAAAGCAGTG |
| Suggested primer 2: | GTCCCTGTATCAACCCCCTT |