| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.209183 |
| Chromosome: | chromosome 1 |
| Location: | 5422369 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038100 | BES1 | (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin); putative chloride channel | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAAAACGCCCGCGGCTGGTGGACTGCCA |
| Internal bar code: | TATACCTGTCGTAGACGCCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 750 |
| LEAP-Seq percent confirming: | 99.532 |
| LEAP-Seq n confirming: | 1914 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAACCCACTACTACCCGA |
| Suggested primer 2: | GTTGAAGCGTGTCTTCCCAT |