| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.209196 |
| Chromosome: | chromosome 11 |
| Location: | 1512467 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467746 | (1 of 4) PF04825 - N terminus of Rad21 / Rec8 like protein (Rad21_Rec8_N) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCAACACGCGCAGCTCCTGCTCCGCAA |
| Internal bar code: | GACCGCTCCAGAGGACTCCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 147 |
| LEAP-Seq percent confirming: | 93.0728 |
| LEAP-Seq n confirming: | 524 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTTGTGCTCCTCAAACAG |
| Suggested primer 2: | TCAGTTTTTCCCCTTGATGC |