| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.209205 |
| Chromosome: | chromosome 17 |
| Location: | 3383413 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723850 | (1 of 1) PF06775 - Putative adipose-regulatory protein (Seipin) (Seipin) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGCATGGAAACGGTCGAGCCAGCTACT |
| Internal bar code: | TGGCATTTGCATGTCCATAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 520 |
| LEAP-Seq percent confirming: | 98.7556 |
| LEAP-Seq n confirming: | 7460 |
| LEAP-Seq n nonconfirming: | 94 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATGATTCAGGTCAGGGAT |
| Suggested primer 2: | CGTAAAACCAACACATTGCG |