| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.209215 |
| Chromosome: | chromosome 17 |
| Location: | 1379266 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g706250 | (1 of 4) PTHR19959:SF128 - KINESIN LIGHT CHAIN 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCTGCGCGTCGCGCGTCATGGCCATAGC |
| Internal bar code: | CAATCGAATCGGGTCCTCGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 152 |
| LEAP-Seq percent confirming: | 13.5158 |
| LEAP-Seq n confirming: | 158 |
| LEAP-Seq n nonconfirming: | 1011 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTGCATGCAAATGGTTTA |
| Suggested primer 2: | ATGAATTGCTGGGGAAGTTG |