| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.209232 |
| Chromosome: | chromosome 14 |
| Location: | 1382305 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617276 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACTACTTGCGCCCTGACGCCCCACCCAG |
| Internal bar code: | AAGACAGGGAGGTTGAGGCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 618 |
| LEAP-Seq percent confirming: | 82.1384 |
| LEAP-Seq n confirming: | 1329 |
| LEAP-Seq n nonconfirming: | 289 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACACCCTAGCACAGCAGA |
| Suggested primer 2: | TCAGTCAACCAGTCAGCAGG |