Insertion junction: LMJ.RY0402.209245_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.209245
Chromosome:chromosome 17
Location:1513430
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g707150 LIC2 Hypothetical protein; (1 of 45) IPR029052 - Metallo-dependent phosphatase-like 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTTGCCTCTCCCCTGCTAGGACATTGGC
Internal bar code:TACACGCGCCGGGACGCCGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:710
LEAP-Seq percent confirming:99.6754
LEAP-Seq n confirming:32246
LEAP-Seq n nonconfirming:105
LEAP-Seq n unique pos:73

Suggested primers for confirmation by PCR

Suggested primer 1:GTCAACCTGCCGTTATCCAT
Suggested primer 2:AGCGAGGGTAAGAGGGAGAG