| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.209257 |
| Chromosome: | chromosome 6 |
| Location: | 3914410 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278183 | (1 of 1) PTHR23054//PTHR23054:SF14 - UNCHARACTERIZED // PROTEIN Y45F10A.7, ISOFORM A | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGATTCTGAGAATGGTGTGTTGTCTAG |
| Internal bar code: | TAAAGTTTTAGATGGCGGATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 307 |
| LEAP-Seq percent confirming: | 99.4773 |
| LEAP-Seq n confirming: | 1713 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGATGAGCTGTGTAGACGA |
| Suggested primer 2: | TATTCCACCGACGGTCTTTC |