| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.209258 |
| Chromosome: | chromosome 7 |
| Location: | 2491302 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329400 | SMM26 | (1 of 89) 3.6.4.13 - RNA helicase; S-adenosyl-L-methionine-dependent methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGGAACTCCTGCACGCGGAGGTGAATG |
| Internal bar code: | TAATCTATCCAGATCACTCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 950 |
| LEAP-Seq percent confirming: | 99.8672 |
| LEAP-Seq n confirming: | 752 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAAGTCGGAGGTGACCAT |
| Suggested primer 2: | GGCCACTCCTTGTCTCTCAG |