Insertion junction: LMJ.RY0402.209261_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.209261
Chromosome:chromosome 8
Location:3528360
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g375250 MRPS19,uS19m (1 of 1) PTHR11880//PTHR11880:SF9 - RIBOSOMAL PROTEIN S19P FAMILY MEMBER // RIBOSOMAL PROTEIN S19, MITOCHONDRIAL; Mitochondrial ribosomal protein S19 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATGCCGCAGCAGCCACACCCGGTCGTCGC
Internal bar code:GCTAACGGCGGGCCTATATATT

Confirmation - LEAP-Seq

LEAP-Seq distance:570
LEAP-Seq percent confirming:98.1501
LEAP-Seq n confirming:1910
LEAP-Seq n nonconfirming:36
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCATGGCAATGTAACAGG
Suggested primer 2:TGGGTATGGAGGTTCAGGAG