| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.209291 |
| Chromosome: | chromosome 6 |
| Location: | 1189338 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g257500 | FTT2 | 14-3-3 protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGATAGTCGTGCTCTAGGCGCACATAC |
| Internal bar code: | TCTGTAGGTTTTAAAAGAATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 462 |
| LEAP-Seq percent confirming: | 99.6317 |
| LEAP-Seq n confirming: | 2164 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGGTAGCGGTGGTAATCG |
| Suggested primer 2: | TACACGGTTGCAGTCTCAGC |