Insertion junction: LMJ.RY0402.209321_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.209321
Chromosome:chromosome 14
Location:2256729
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g623150 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACAGACCATTACAAACCCAGAACGCATAG
Internal bar code:GTAGGAGGCAGCGGTGAATGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:709
LEAP-Seq percent confirming:99.7775
LEAP-Seq n confirming:1345
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTTTAGTTTGGGCTGGTA
Suggested primer 2:CGCGAACTCAGCCCTACTAC