| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.209350 |
| Chromosome: | chromosome 3 |
| Location: | 3645251 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g168800 | KIN4-1,KIN4A | (1 of 2) PTHR24115//PTHR24115:SF136 - FAMILY NOT NAMED // KLP31E, ISOFORM A; Kinesin motor protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTCCAGGAGCAATGCAGCTGCCATGCAC |
| Internal bar code: | AGGGTTCCGGGATGGCGCGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 407 |
| LEAP-Seq percent confirming: | 99.769 |
| LEAP-Seq n confirming: | 4751 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAGCAACTCCACCTACGG |
| Suggested primer 2: | ACACACACTCACGGCAGAAA |