Insertion junction: LMJ.RY0402.209355_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.209355
Chromosome:chromosome 15
Location:421722
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre15.g636250 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGAGTACAACATGCATACCCGCCCGCACC
Internal bar code:CGCGACAGGAGGGGCTAGGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:516
LEAP-Seq percent confirming:99.3727
LEAP-Seq n confirming:2376
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTCAACACCCCAATACCC
Suggested primer 2:GAGAGGTCCCAGGAGGGTAG