| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.209376 |
| Chromosome: | chromosome 6 |
| Location: | 4154252 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278229 | (1 of 3) IPR000104//IPR004333 - Antifreeze protein, type I // Transcription factor, SBP-box | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGCGGCTGCGTACTGCTGCGAGCCGTAG |
| Internal bar code: | TCTCCGGCTGTCCCTTCAGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 290 |
| LEAP-Seq percent confirming: | 99.6603 |
| LEAP-Seq n confirming: | 1467 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGTGTGAAAACCATTTG |
| Suggested primer 2: | ATGTACCACTAGCGCCTGCT |