| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.209377 |
| Chromosome: | chromosome 16 |
| Location: | 3510809 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676314 | EIF3H | Eukaryotic translation initiation factor 3, subunit H | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTATGGCCGCGTTGAAGCACACACAGCT |
| Internal bar code: | GGGATCTCGGGGAACGGAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 660 |
| LEAP-Seq percent confirming: | 99.5459 |
| LEAP-Seq n confirming: | 4823 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCGACTCAACCTGTCCTC |
| Suggested primer 2: | AGTGCGCATGTGTTGAATGT |