| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.209381 |
| Chromosome: | chromosome 1 |
| Location: | 5060549 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035000 | (1 of 1) K14791 - periodic tryptophan protein 1 (PWP1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGCGAGATAAGCATGATTGCCCGAGGCC |
| Internal bar code: | GGTTATAGGATTGGAATGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 209 |
| LEAP-Seq percent confirming: | 99.3124 |
| LEAP-Seq n confirming: | 5055 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTTACCCCAATTGCAGGC |
| Suggested primer 2: | ATTACTTGTGGGGACAACGG |