| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.209396 |
| Chromosome: | chromosome 3 |
| Location: | 5048453 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g180850 | SEC23A | (1 of 1) K14006 - protein transport protein SEC23 (SEC23); COP-II coat subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCTGCCTTAGACGGTGCAGCATACTCGA |
| Internal bar code: | GCGTCCGGCGTTGAGTATGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 578 |
| LEAP-Seq percent confirming: | 99.627 |
| LEAP-Seq n confirming: | 3739 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTACGTGGTGGTGTTCCA |
| Suggested primer 2: | AGTCCTCGAACAGTCGCAGT |