| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.209416 |
| Chromosome: | chromosome 6 |
| Location: | 2639819 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g269865 | SMM18 | (1 of 1) IPR000104//IPR029044 - Antifreeze protein, type I // Nucleotide-diphospho-sugar transferases; S-adenosyl-L-methionine-dependent methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGTGCCCGGCGGCTCTGCTTCTGCTGC |
| Internal bar code: | ACTGCAGTGGAGGGATATCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 341 |
| LEAP-Seq percent confirming: | 86.3388 |
| LEAP-Seq n confirming: | 158 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATTACACCCGACAGCTCC |
| Suggested primer 2: | CCAAGCATCCAAACACACAC |