Insertion junction: LMJ.RY0402.209424_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.209424
Chromosome:chromosome 3
Location:5904596
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g190000 PPP14 Phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCTGACCACACGACTGCGCATCCACAGC
Internal bar code:ACAACTACGTCAGAGGTCGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:705
LEAP-Seq percent confirming:99.4672
LEAP-Seq n confirming:2427
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CTTTGTATGATGTGGCACCG
Suggested primer 2:AACCCTGGCACCTATCACTG