| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.209432 |
| Chromosome: | chromosome 17 |
| Location: | 162328 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g697300 | CEP16 | Cysteine endopeptidase; (1 of 1) 2.7.11.1//3.4.22.67 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Zingipain / Zingibain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAAGCCAGCATGTAAGACGTCGTGGCGGG |
| Internal bar code: | GGGAGCTAGCGGATGCCTGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1056 |
| LEAP-Seq percent confirming: | 99.7823 |
| LEAP-Seq n confirming: | 5500 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAACAGCTGCGCATTTAC |
| Suggested primer 2: | TTGGGTCTACTTCACGGTCC |