| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.209480 |
| Chromosome: | chromosome_3 |
| Location: | 7975451 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g201900 | RSP1 | Radial spoke protein 1 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCTACAAAGGATGGGGTTGCATGCCGCATG |
| Internal bar code: | GCTTTCTCTACCACGGCTCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 547 |
| LEAP-Seq percent confirming: | 99.7069 |
| LEAP-Seq n confirming: | 3742 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTCGCAGCCCTTCCTAAA |
| Suggested primer 2: | TGTCGCCTCCTACCAATACC |