| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.209486 |
| Chromosome: | chromosome 5 |
| Location: | 2805654 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g237250 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCTTTCCTGATGGCCGCCCTACCCAAAT |
| Internal bar code: | CGTGGAGGCCAATTTAGCTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1010 |
| LEAP-Seq percent confirming: | 99.1668 |
| LEAP-Seq n confirming: | 6665 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCTCGTTCAACATGCTCG |
| Suggested primer 2: | CTAGGACAGCCATAGCCTGC |