| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.209488 |
| Chromosome: | chromosome 5 |
| Location: | 647656 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g231050 | (1 of 1) K10636 - autocrine motility factor receptor (AMFR, GP78) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTAAGAACTAAGCTCCGAAGGGCTCCAG |
| Internal bar code: | GGCAGGTGGAACGATAAATTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 350 |
| LEAP-Seq percent confirming: | 99.3605 |
| LEAP-Seq n confirming: | 3263 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTGCCTCTTATCCTGAAC |
| Suggested primer 2: | CTCTATCTCGCCGTACCAGC |