| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.209523 |
| Chromosome: | chromosome 4 |
| Location: | 209761 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217220 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTGTATGTGTGTCTGTACGACAAGCTG |
| Internal bar code: | TGACTTGATTCAGCGGGTGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 671 |
| LEAP-Seq percent confirming: | 98.3456 |
| LEAP-Seq n confirming: | 1605 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTTTGGGCTGGAATTTAC |
| Suggested primer 2: | GCGACTGCTAGATAGGCACC |