| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.209542 |
| Chromosome: | chromosome 5 |
| Location: | 996242 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246650 | (1 of 1) IPR023214//IPR027377 - HAD-like domain // Zinc-binding domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGCAAAGGAAACAAGATGCAGATGAAGT |
| Internal bar code: | GTGCGACACTTCTATTCGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1001 |
| LEAP-Seq percent confirming: | 99.7809 |
| LEAP-Seq n confirming: | 911 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAAGCTGGGCTATTCCTG |
| Suggested primer 2: | TGGTGGTGGTGATAGTGGTG |