| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.209542 |
| Chromosome: | chromosome_5 |
| Location: | 996286 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g246650 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTGGCACTCAACACCAACTACAGCTATGA |
| Internal bar code: | AGGCCGTACGTAGACGTGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 759 |
| LEAP-Seq percent confirming: | 99.8043 |
| LEAP-Seq n confirming: | 2040 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAAGCTGGGCTATTCCTG |
| Suggested primer 2: | TGGTGGTGGTGATAGTGGTG |