Insertion junction: LMJ.RY0402.209550_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.209550
Chromosome:chromosome 17
Location:181240
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g697406 (1 of 45) IPR029052 - Metallo-dependent phosphatase-like CDS/intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGCAGCGCCGGCGACGATGTCGCAGCGC
Internal bar code:GTGGACCTCTCCGGTCGCACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:318
LEAP-Seq percent confirming:99.6038
LEAP-Seq n confirming:2514
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CATTCGCAGGATGATGTGAC
Suggested primer 2:GGAGCTCAAGCAGGGTGTAG