| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.209563 |
| Chromosome: | chromosome_12 |
| Location: | 2695503 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g503800 | RMT5 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCTTCCTGGCTACGCCTTCACTTCTTAGAT |
| Internal bar code: | TGCAGGGATGCTAGGCATGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 364 |
| LEAP-Seq percent confirming: | 96.5217 |
| LEAP-Seq n confirming: | 333 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTACCAGTACCCTGCCTGG |
| Suggested primer 2: | CATCAGCCCACTACTGCTCA |