Insertion junction: LMJ.RY0402.209571_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.209571
Chromosome:chromosome 16
Location:1917952
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g656050 (1 of 26) IPR000209 - Peptidase S8/S53 domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTTATGGAGCATCTTCCGGTCCTTCTTT
Internal bar code:TGGGCTGTACTCTCGCGATCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:910
LEAP-Seq percent confirming:97.387
LEAP-Seq n confirming:1379
LEAP-Seq n nonconfirming:37
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CACCAACAGCTGATGTACGG
Suggested primer 2:CCGTGTAAGTGGGTTTTGCT