| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.209576 |
| Chromosome: | chromosome_3 |
| Location: | 7976164 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g201900 | RSP1 | Radial spoke protein 1 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGTCCATCCGCCCTCCGCCTGCATTCATC |
| Internal bar code: | CTATAGGCCCATTCCGGTGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 930 |
| LEAP-Seq percent confirming: | 99.7337 |
| LEAP-Seq n confirming: | 1498 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAAGGTCCAGCTCAGGTC |
| Suggested primer 2: | AATAGGAGACTGGCACACCG |