Insertion junction: LMJ.RY0402.209631_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.209631
Chromosome:chromosome 16
Location:226359
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g694704 (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGCGCCGGTTCCTTTCCTTTCCGGGTTG
Internal bar code:TCAAATCGCAGGTTCACCACAC

Confirmation - LEAP-Seq

LEAP-Seq distance:569
LEAP-Seq percent confirming:97.4359
LEAP-Seq n confirming:76
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGGACGAGCTGATGTACGAG
Suggested primer 2:CGGGTACATTACCCACATCC